The Rockefeller University Proteomics Resource Center

News & Announcements

General PRC news items and announcements will appear on this page. For news about a particular department link to that department from the PRC Home Page.

The New UltiMate™ 3000 Intelligent nano-HPLC system has been installed. A method to conduct 2-dimensional liquid chromatography (2D LC-MS/MS or MudPIT) analysis has been established. MudPIT stands for Multidimensional Protein Identification Technology that is used for the separation and identification of complex protein mixtures. The MudPIT technique involves digesting the complex protein sample into its constituent peptides in solution, which are then separated with two liquid column chromatography steps: The first being a strong cationic exchange, and the second being reversed-phase high performance liquid chromatography (HPLC). MudPIT has been used to analyze the proteomes of organisms without gel electrophoresis separation.

The AKTA system from Amersham Pharmacia Biotech (GE Healthcare) is currently in service. The AKTA System is an automated chromatographic system that can be utilized in purification of proteins by ion-exchange, size exclusion, hydrophobic interaction, and affinity chromatography.

A new microwave-assisted peptide synthesizer has been installed at the center that allows us to make difficult peptides and shorten the turn around time. For most custom peptides, the turn around time is within two weeks (10 working days). For rush order, peptides will be delivered within a week (5 working days). Please contact Mouli @8487 or chandrn@rockefeller.edu or Henry zebrosh@mail.rockefeller.edu . We are also available for consultation if you need any assistance in designing a peptide.

An Intavis Multipep robotic peptide synthesizer has been recently installed. This instrument is capable of making up to 192 peptides at a time either as free peptides or membrane bound. This system is ideal for the rapid synthesis of a large number of moderate length peptides at smaller scales (several milligrams). Come on down to see it or contact us for more information.

Check out the new, simplified, lower pricing on our mass spectrometry based proteomics services!

Our Typhoon 9200 imager has been upgraded to a Typhoon 9400 imager with red, green and blue lasers. The Typhoon 9400 is capable of full DIGE scanning of three color fluorescent electrophoresis gels as well as analysis of storage phosphor plates, general fluorescence detection of gels, blots and plates and chemiluminescence detection. For questions about gel electrophoresis and Typhoon 9400 system, please contact Dr Haiteng Deng @7525 or dengh@rockefeller.edu.

The page last updated on April 28th, 2007

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