Mass Spectrometry 3. Charges

The Rockefeller University Proteomics Resource Center

Mass Spectrometry Service and Fees

Summary of the Mass Spectrometry Service and in-house fee schedule (outside investigators will incur a 25% surcharge). The Service operates on a first come, first served basis. The Service will archive data for a maximum of 6 months after which the data may be deleted. We accept outside samples from non-profit institutions but be advised that in-house investigators are given a higher priority and there is a 25% surcharge in addition to the prices given below. Contact Mass Spectrometry at (212)327-7525 or (212)327-8869 (click here for email) before submitting your sample in order to determine the best strategy for analysis of your specific sample.

In addition to the routine service outlined below, more extensive or complex projects (proteomics, protein modifications, etc.) are welcomed on a collaborative and time available basis. Please contact Dr. Haiteng Deng for more information and pricing.

Sample submittal instructions

IDENTIFICATION OF PROTEINS FROM 1D/2D SDS PAGE

Samples can be submitted as gels or excised gel bands
A combination of in-gel digestion, LC/MS/MS and database searching is used for identification of proteins.
$225 ($270 for external users) for the first sample and $83 ($104 for external users) for each additional sample

IDENTIFICATION OF PROTEIN POST-TRANSLATION MODIFICATIONS

Samples can be submitted as gels or excised gel bands or solution
A combination of in-gel digestion, LC/MS/MS and database searching is used for identification of proteins.
$225 ($270 for external users) for the digestion and $332 ($416 for external users) for PTM.

SAMPLE PREPARATION

Reversed-phase HPLC purification (0.3 or 1 mm columns) with fraction collection: $225 ($270 for external users)
Sample cleanup (desalting, concentration):$28 ($35 for external users)

MALDI-TOF MS

Purified or synthetic peptide mass determination using alpha cyano-4-hydroxycinnamic acid (HCCA) or 2,5 dihydroxy benzoic acid (DHB), in positive or negative mode:
- External calibration: $33 ($42 for external users)
- Internal calibration: $83 ($104 for external users)
Intact protein mass determination using alpha cyano-4-hydroxycinnamic acid (HCCA) or sinapinic acid (SA):
- External calibration: $45 ($57 for external users)
- Internal calibration: $108 ($135 for external users)

ESI MS

Small molecule mass determination (no interpretation)
- Infusion: $68 ($88 for external users)
- LC-MS or MS/MS: $280 ($353 for external users)

MISCELLANEOUS

Enzymatic dephosphorylation: $28 ($36 for external users)
Conversion of data to PDF format: $28 ($36 for external users)
Storage of data to CD-ROM: $28 ($36 for external users)
Data conversion to publication quality spectrum: $28 ($36 for external users)

Sample submittal and payment instructions

All investigators must complete a submittal form that can be downloaded as a PDF file (Download here). Please do not fill in the grayed out areas
In-house investigators must provide an account number and the primary investigator’s (or other authorized) signature on the form.
Outside investigators must submit a valid purchase order to the Rockefeller University Proteomics Resource Center from their non-profit organization, credit card or bank transfer information before a sample will be accepted for analysis
Click here for sample preparation recommendations

This page last updated on July 17th, 2008

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